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Product Information

Malolactic Cultures

Malo-lactic Cultures Product Information and Usage

Wyeast Laboratories, Inc. Malo-lactic Cultures are designed for direct inoculation of 6 gallons of wine.

Malo-lactic Culture packages contain a live liquid suspension of Oenococcus oenii grown in a sterile organic juice based nutrient medium. Malic acid reduction will balance and soften wine while enhancing flavor and aroma characteristics including vanilla and buttery notes. 4007 Blend (blend of ER1A and EY2d cultures) will provide rapid and complete malic acid reduction in wine over a broad spectrum of conditions. ER1A, an excellent choice for red wines, has been isolated for it’s tolerance to low pH coditions. Ey2D has been selected for it tolerance to low cellar temperatures. Malo-lactic conversion is generally completed within 1-3 months.


The Malo-lactic Culture packages contain 125 ml of slurry. This provides the inoculation rate recommended by and for professional vintners for 6 gallons of wine. These cultures will convert malic acid to lactic acid over a broad temperature range (55-75°F). The Malo-lactic cultures will also perform over a broad spectrum of pH conditions. Inoculate wine that has completed or nearly completed alcoholic fermentation. Free SO2 levels should be less than 15 ppm.

Basic instructions for the proper use of Malo-lactic packages:

  1. Shake well and sanitize package
  2. Open and pour into 6 gallons of wine or must that has completed or nearly completed alcoholic fermentation
  3. Maintain fermentation or cellaring temperature between 55-75°F (13-24°C). Malo-lactic fermentation is generally complete within 1-3 months.

Please see the ML Propagation section of this site for information on expanding your ML culture.

Sterile Packaged Product – UV Light Barrier – 100% Guaranteed Pure
Refrigerate between 34-40°F (1-4°C). 
Do not freeze.

Best if Used by: This package is best when used within 6 months of the manufacturer’s date when stored between 34-40°F (1-4°C). Older malo-lactic cultures that have been exposed to higher or lower temperatures may perform slowly.


Our Product Warranty states that we guarantee the viability of the product in our Malo-lactic culture packages for 6 months from the manufacture date assuming that they have been properly shipped, stored and handled. Our superior packaging material provides 100% oxygen barrier and UV light protection making this exceptional guarantee possible. During this 6 month guaranteed shelf life, some loss of viability is to be expected.

Malo-lactic culture packages will sometimes swell slightly, or moderately during shipping or later while properly stored. This is not an indication of deterioration if the package is less than 6 months old and has been properly handled. This is result of trace amounts of nutrients, still available at the time packaging, causing small amount of culture activity and CO2 production.

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